Sohn K H, Lee H Y, Chung H Y, Young H S, Yi S Y, Kim K W
Department of Molecular Biology, Pusan National University, Korea.
Cancer Lett. 1995 Aug 1;94(2):213-8. doi: 10.1016/0304-3835(95)03856-r.
Ursolic acid (UA) and oleanolic acid (OA) were examined for anti-angiogenic activities by using the chick embryo chorioallantoic membrane (CAM) assay. The presence of UA or OA inhibited angiogenesis in a dose-dependent manner; the doses required for half-maximal inhibition (ID50) were 5 micrograms and 40 micrograms per CAM, respectively. UA was a more potent angiogenic inhibitor than OA. We also tested for inhibitory effect on the proliferation of bovine aortic endothelial cell. They effectively inhibited the proliferation of bovine aortic endothelial cell in a concentration-dependent manner. The IC50 values of anti-proliferative effects were determined to be 5 microM for UA and 20 microM for OA. Based on these results, we speculated that the inhibitory effects on bovine aortic endothelial cell proliferation of UA and OA might be important for anti-angiogenesis.
通过鸡胚绒毛尿囊膜（CAM）试验检测了熊果酸（UA）和齐墩果酸（OA）的抗血管生成活性。UA或OA的存在以剂量依赖性方式抑制血管生成；半最大抑制所需剂量（ID50）分别为每CAM 5微克和40微克。UA是比OA更有效的血管生成抑制剂。我们还测试了对牛主动脉内皮细胞增殖的抑制作用。它们以浓度依赖性方式有效抑制牛主动脉内皮细胞的增殖。UA抗增殖作用的IC50值确定为5 microM，OA为20 microM。基于这些结果，我们推测UA和OA对牛主动脉内皮细胞增殖的抑制作用可能对抗血管生成很重要。
