Sandi C, Venero C, Guaza C
Psychobiology Research Group, Cajal Institute, CSIC, Madrid, Spain.
Eur J Pharmacol. 1995 Apr 13;277(1):89-97. doi: 10.1016/0014-2999(95)00068-v.
In the central nervous system, nitric oxide has been proposed to be a retrograde messenger mediating learning and synaptic plasticity. Since only pretraining injections of nitric oxide synthesis inhibitors were shown to impair learning, we examined the possibility that systemic administration of these inhibitors might influence some non-specific aspects related to the organism's general psychophysiological status. Intraperitoneal administration of NG-nitro-L-arginine methyl ester (30 or 100 mg/kg) 60 min pre-test to adult rats resulted in: (i) altered exploratory pattern and reduced locomotion in a novel environment; (ii) reduced startle response to either acoustic or electric stimuli; and (iii) cardiovascular alterations. In addition, intracerebroventricular administration of N-nitro-L-arginine (10 microliters of a 10 mM solution) diminished the acoustic startle response. Specificity of these effects through nitric oxide was supported by the ability of the nitric oxide precursor, L-arginine, to prevent the inhibitors actions. These findings indicate that nitric oxide inhibitors interfere with the general psychophysiological status of the organism.
在中枢神经系统中，一氧化氮被认为是一种逆行信使，介导学习和突触可塑性。由于只有在训练前注射一氧化氮合成抑制剂才会损害学习，我们研究了全身给予这些抑制剂可能影响与生物体一般心理生理状态相关的一些非特异性方面的可能性。在成年大鼠测试前60分钟腹腔注射NG-硝基-L-精氨酸甲酯（30或100毫克/千克）导致：（i）在新环境中探索模式改变和运动减少；（ii）对声音或电刺激的惊吓反应降低；以及（iii）心血管改变。此外，脑室内注射N-硝基-L-精氨酸（10微升10毫摩尔溶液）减少了声音惊吓反应。一氧化氮前体L-精氨酸预防抑制剂作用的能力支持了这些通过一氧化氮产生的效应的特异性。这些发现表明一氧化氮抑制剂会干扰生物体的一般心理生理状态。
