Schraven B, Peter M E
Division of Applied Immunology, German Cancer Research Center, Heidelberg.
FEBS Lett. 1995 Jul 24;368(3):491-4. doi: 10.1016/0014-5793(95)00720-t.
Tyrosine phosphorylation has been reported to be an early event required for APO-1/Fas(CD95) signalling in lymphocytes [Eischen, C.M., Dick, C.J. and Leibson, P.J. (1994) J. Immunol. 153, 1947-1954]. We have compared two mutant Jurkat cells, one largely deficient in expression of CD-45 (J45.01) and a second one deficient in expression of p56lck (JCaM1.6) with wild type Jurkat cells for their ability to undergo APO-1-induced apoptosis. No significant difference was observed among the three cell lines. In the mutant Jurkat cells APO-1 triggering did not result in increased tyrosine phosphorylation of cytosolic proteins. Furthermore, herbimycin A did not inhibit but rather augmented apoptosis at concentrations which effectively degraded the src related kinases lck and fyn. The data suggest that APO-1-mediated signalling is independent from src kinases and CD45.
据报道，酪氨酸磷酸化是淋巴细胞中APO-1/Fas(CD95)信号传导所需的早期事件[艾申，C.M.，迪克，C.J.和莱布森，P.J.（1994年）《免疫学杂志》153，1947 - 1954]。我们将两种突变的Jurkat细胞与野生型Jurkat细胞进行了比较，一种在很大程度上缺乏CD-45的表达（J45.01），另一种缺乏p56lck的表达（JCaM1.6），比较它们经历APO-1诱导的凋亡的能力。在这三种细胞系之间未观察到显著差异。在突变的Jurkat细胞中，APO-1触发并未导致胞质蛋白酪氨酸磷酸化增加。此外，除莠霉素A在有效降解src相关激酶lck和fyn的浓度下，并未抑制反而增强了凋亡。数据表明，APO-1介导的信号传导独立于src激酶和CD45。
