枯草芽孢杆菌中trpG的翻译受色氨酸RNA结合衰减蛋白（TRAP）调控。
Translation of trpG in Bacillus subtilis is regulated by the trp RNA-binding attenuation protein (TRAP).
作者信息
Yang M, de Saizieu A, van Loon A P, Gollnick P
机构信息
Department of Biological Sciences, University of New York at Buffalo 14260, USA.
出版信息
J Bacteriol. 1995 Aug;177(15):4272-8. doi: 10.1128/jb.177.15.4272-4278.1995.
Abstract
trpG of Bacillus subtilis encodes a glutamine amidotransferase subunit that is involved in the synthesis of both folic acid and L-tryptophan. Expression of trpG is negatively regulated by tryptophan even though this gene is located within a folic acid biosynthetic operon. Examination of both transcriptional and translational gene fusions to lacZ involving trpG and direct measurements of trpG mRNA levels and TrpG polypeptide accumulation demonstrated that translation of trpG is regulated by tryptophan whereas transcription is not. these studies also show that this regulation is mediated by the trp RNA-binding attenuation protein. Deletion and point mutations indicated that regulation is dependent on a series of G/UAG trinucleotide repeats surrounding the putative ribosome-binding site for trpG. Our results are consistent with a model in which the tryptophan-activated trp RNA-binding attenuation protein and ribosomes compete for binding to trpG mRNA.
摘要
枯草芽孢杆菌的trpG编码一种谷氨酰胺酰胺转移酶亚基，该亚基参与叶酸和L-色氨酸的合成。尽管trpG基因位于一个叶酸生物合成操纵子内，但其表达受到色氨酸的负调控。对涉及trpG的lacZ转录和翻译基因融合的研究以及对trpG mRNA水平和TrpG多肽积累的直接测量表明，trpG的翻译受色氨酸调控，而转录不受其调控。这些研究还表明，这种调控是由trp RNA结合衰减蛋白介导的。缺失和点突变表明，调控依赖于围绕trpG假定核糖体结合位点的一系列G/UAG三核苷酸重复序列。我们的结果与一个模型一致，在该模型中，色氨酸激活的trp RNA结合衰减蛋白和核糖体竞争与trpG mRNA的结合。
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