大肠杆菌16S核糖体RNA中特定G-C碱基对被A-U取代相关的生长特性
Growth properties associated with A-U replacement of specific G-C base pairs in 16S rRNA from Escherichia coli.
作者信息
Triman K L
机构信息
Department of Biology, Franklin and Marshall College, Lancaster, Pennsylvania 17604, USA.
出版信息
J Bacteriol. 1995 Aug;177(15):4514-6. doi: 10.1128/jb.177.15.4514-4516.1995.
Abstract
Mutations that disrupt each of seven specific G-C base pairs in 16S rRNA from Escherichia coli confer loss of expression of a plasmid-encoded 16S rRNA selectable marker (spectinomycin resistance). However, A-U replacement of G-C base pairs at nucleotides 359/52 or 1292/1245 in 16S rRNA permits normal expression of the marker. By contrast, A-U replacements at 146/176, 153/168, 350/339, or 1293/1244 are associated with loss of expression of the marker. These genetic studies are designed to determine the importance of specific base pairs by assessment of the structural and functional impairments of 16S rRNA molecules resulting from expression of base pair substitutions at these positions.
摘要
破坏大肠杆菌16S rRNA中七个特定G-C碱基对的突变会导致质粒编码的16S rRNA选择标记（壮观霉素抗性）表达缺失。然而，16S rRNA中核苷酸359/52或1292/1245处的G-C碱基对被A-U取代可使该标记正常表达。相比之下，146/176、153/168、350/339或1293/1244处的A-U取代与该标记表达缺失有关。这些遗传学研究旨在通过评估这些位置的碱基对取代表达所导致的16S rRNA分子的结构和功能损伤，来确定特定碱基对的重要性。
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