Zientara S, Sailleau C, Moulay S, Wade-Evans A, Cruciere C
CNEVA-Laboratoire Central de Recherches Vétérinaires, Maisons-Alfort, France.
J Virol Methods. 1995 May;53(1):47-54. doi: 10.1016/0166-0934(94)00175-g.
The development of a coupled reverse transcriptase-polymerase chain reaction assay (RT-PCR) is described for the detection of African horse sickness virus (AHSV) double-stranded RNA. Genome segments 7 and 10 were chosen as target templates for primers selected for use in the RT-PCR. Using these AHSV-specific primers all 9 serotypes were detectable. The sensitivity and specificity of the RT-PCR results were compared to those obtained by competition ELISA.
