Chen L, Ma C, Cai B C, Lu Y M, Wu H
Institute of TCM, Nanjing College of Traditional Chinese Medicine.
Yao Xue Xue Bao. 1995;30(3):168-71.
Ventricular myocardiocytes from neonatal Wistar rats were isolated and cultured. Aconitine, Ca2+ channel blocker verapamil or Ca2+ channel activator BAY K8644 were added to the bath solution separately. Using the cell-attached configuration of the patch clamp technique, the single channel activities of L type Ca2+ channel were recorded before and after addition of all three drugs. The results showed the blocking effect of aconitine (50 micrograms.ml-1) on L type Ca2+ channels. Its mechanism may be relevant to the decrease in both open state probability and the mean open time of Ca2+ channel. The difference was statistically significant compared with control group (P < 0.01). The amplitude of Ba2+ currents, which flow through open L type Ca2+ channel was unchanged.
分离并培养新生Wistar大鼠的心室肌细胞。将乌头碱、Ca2+通道阻滞剂维拉帕米或Ca2+通道激活剂BAY K8644分别添加到浴液中。采用膜片钳技术的细胞贴附式记录这三种药物添加前后L型Ca2+通道的单通道活性。结果显示乌头碱（50微克·毫升-1）对L型Ca2+通道有阻断作用。其机制可能与Ca2+通道开放概率和平均开放时间的降低有关。与对照组相比差异有统计学意义（P<0.01）。流经开放L型Ca2+通道的Ba2+电流幅度未改变。
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