鸡生长激素编码基因内含子1中存在一个额外的PstI片段。

Presence of an additional PstI fragment in intron 1 of the chicken growth hormone-encoding gene.

作者信息

Mou L, Liu N, Zadworny D, Chalifour L, Kuhnlein U

出版信息

Gene. 1995 Jul 28;160(2):313-4. doi: 10.1016/0378-1119(95)96895-8.

Abstract

A previously unreported 196-bp PstI fragment was found in intron 1 of the gene encoding chicken growth hormone (cGH) when a PCR assay for an MspI restriction fragment length polymorphism was established. A pair of PCR primers was designed according to the published cGH sequence and used to amplify a fragment which contained two MspI sites, one polymorphic and another non-polymorphic. However, amplification of genomic DNA from two strains of meat-type chickens and three strains of White Leghorn chickens yielded a PCR product which was about 200 bp larger than expected. The fragment from one of the meat-type chickens was subcloned into the vector pCR-Script SK+, and sequenced. It revealed the presence of an extra fragment of 196 bp which was flanked by the PstI sites and occurred at nt +308 of the previously reported cGH sequence.

摘要

在建立鸡生长激素（cGH）编码基因内含子1的MspI限制性片段长度多态性的PCR检测方法时，发现了一个先前未报道的196 bp PstI片段。根据已发表的cGH序列设计了一对PCR引物，用于扩增一个包含两个MspI位点的片段，其中一个位点具有多态性，另一个位点无多态性。然而，从两株肉用型鸡和三株白来航鸡的基因组DNA扩增得到的PCR产物比预期的大约200 bp。将其中一株肉用型鸡的片段亚克隆到载体pCR-Script SK+中并进行测序。结果显示存在一个196 bp的额外片段，其两侧为PstI位点，位于先前报道的cGH序列的nt +308处。