Minamoto A, Sakata H, Okada K, Fujihara M
Department of Ophthalmology, Hiroshima University School of Medicine, Japan.
Jpn J Ophthalmol. 1995;39(1):12-9.
The usefulness of the subconjunctival injection of FK-506 for suppression of allograft rejection was investigated in a rat model of orthotopic penetrating keratoplasty. Fischer rats were used as donors and Dark Agouti rats, as recipients. FK-506 was administered subconjunctivally in a dosage of 0.3 mg/kg/day for 15 consecutive days after penetrating keratoplasty was performed. Allograft rejection occurred within 8 to 10 days after keratoplasty in all untreated rats (n = 6). None of the FK-506-treated rats (n = 6) exhibited graft rejection during the 3-week observation period. Histologic examination showed marked infiltration of mononuclear cells in the stroma of corneal grafts from untreated rats 3 weeks after grafting. Inflammatory cells were only occasionally observed in grafts from FK-506-treated rats. Donor-specific cytotoxic T lymphocyte activity was completely suppressed in FK-506-treated rats 3 weeks after grafting. Our results indicated that subconjunctival injection of FK-506 effectively prevented corneal allograft rejection in a rat model of penetrating keratoplasty.
在原位穿透性角膜移植大鼠模型中，研究了结膜下注射FK - 506对抑制同种异体移植排斥反应的有效性。以Fischer大鼠作为供体，深色刺豚鼠作为受体。在进行穿透性角膜移植术后，连续15天每天以0.3 mg/kg的剂量结膜下注射FK - 506。所有未治疗的大鼠（n = 6）在角膜移植术后8至10天内发生同种异体移植排斥反应。在3周的观察期内，所有接受FK - 506治疗的大鼠（n = 6）均未出现移植排斥反应。组织学检查显示，移植3周后，未治疗大鼠角膜移植片基质中有明显的单核细胞浸润。在接受FK - 506治疗的大鼠的移植片中仅偶尔观察到炎性细胞。移植3周后，FK - 506治疗的大鼠的供体特异性细胞毒性T淋巴细胞活性被完全抑制。我们的结果表明，结膜下注射FK - 506可有效预防穿透性角膜移植大鼠模型中的角膜同种异体移植排斥反应。
