Takenaka K, Suzuki S, Sakai N, Kassell N F, Yamada H
Department of Neurosurgery, Gifu University School of Medicine, Japan.
Biochem Biophys Res Commun. 1995 Aug 15;213(2):608-15. doi: 10.1006/bbrc.1995.2175.
Incubation with holo-transferrin, but not apo-transferrin, induced the nitrite accumulation in the culture medium of rat aortic smooth muscle cells concentration- and time-dependently, which occurred after a lag period of 6 hours. The accumulation of nitrite was inhibited by N omega-nitro-L-arginine and cycloheximide, but not by calmidazolium. Ferric or ferrous iron had no effect on the accumulation. In reverse transcription-coupled polymerase chain reaction analysis, one amplified fragment (580 bp) corresponding to the inducible form of nitric oxide synthase (iNOS) mRNA was expressed after incubation with holo-transferrin for 10 hours. These results suggest that holo-transferrin activates expression of iNOS mRNA in rat aortic smooth muscle cells accompanied by nitric oxide accumulation via a pathway dependent on L-arginine in the culture medium.
与全转铁蛋白而非脱铁转铁蛋白共同孵育，能浓度和时间依赖性地诱导大鼠主动脉平滑肌细胞培养基中亚硝酸盐积累，这一过程在6小时的延迟期后出现。亚硝酸盐的积累受到Nω-硝基-L-精氨酸和放线菌酮的抑制，但不受钙调蛋白的抑制。三价铁或二价铁对积累无影响。在逆转录偶联聚合酶链反应分析中，与全转铁蛋白孵育10小时后，出现了一个与诱导型一氧化氮合酶（iNOS）mRNA相对应的扩增片段（580 bp）。这些结果表明，全转铁蛋白通过依赖培养基中L-精氨酸的途径，激活大鼠主动脉平滑肌细胞中iNOS mRNA的表达并伴随一氧化氮积累。
