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A rapid RT-PCR method for detection of intact RNA in formalin-fixed paraffin-embedded tissues.

作者信息

Jiang Y H, Davidson L A, Lupton J R, Chapkin R S

机构信息

Faculty of Nutrition, Molecular and Cell Biology Group, Texas A&M University, College Station 77843-2471, USA.

出版信息

Nucleic Acids Res. 1995 Aug 11;23(15):3071-2. doi: 10.1093/nar/23.15.3071.

DOI:10.1093/nar/23.15.3071
PMID:7544892
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC307153/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df30/307153/329e943e163b/nar00015-0279-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df30/307153/5ce7e6371457/nar00015-0279-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df30/307153/329e943e163b/nar00015-0279-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df30/307153/5ce7e6371457/nar00015-0279-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df30/307153/329e943e163b/nar00015-0279-b.jpg

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Am J Pathol. 1994 Apr;144(4):659-66.
2
Protein kinase C isoforms in human and rat colonic mucosa.人和大鼠结肠黏膜中的蛋白激酶C亚型
Arch Biochem Biophys. 1994 Aug 1;312(2):547-53. doi: 10.1006/abbi.1994.1344.
3
Preparation of crude cell extract suitable for amplification of RNA by the polymerase chain reaction.制备适用于通过聚合酶链反应扩增RNA的粗细胞提取物。
Nucleic Acids Res. 1989 Mar 11;17(5):2141. doi: 10.1093/nar/17.5.2141.
4
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.采用酸性硫氰酸胍-苯酚-氯仿萃取法一步分离RNA的方法。
Anal Biochem. 1987 Apr;162(1):156-9. doi: 10.1006/abio.1987.9999.
5
Molecular pathology of paraffin-embedded tissue. Current clinical applications.
Diagn Mol Pathol. 1992 Sep;1(3):206-11.