[来自大脑的单胺氧化酶A内源性抑制剂（刺蒺藜素A）：纯化与结构鉴定]

[An endogenous inhibitor of monoamine oxidase A (tribulin A) from brain: purification and structure identification].

作者信息

Medvedev A E, Kamyshanskaia N S, Halket J, Glover V, Sandler A

机构信息

B. Baron Memorial Laboratories, Department of Chemical Pathology, Queen Charlotte and Chelsea Hospial, London, U.K.

出版信息

Biokhimiia. 1995 May;60(5):659-67.

PMID:7545013

Abstract

The endogenous monoamine oxidase inhibitor, tribulin, contains several components which selectively (or nonselectively) inhibit monoamine oxidases A and B. The pig brain tribulin component selectively inhibiting monoamine oxidase A was purified and identified as 4-hydroxyphenylethanol using gas chromatography-mass spectrometry. This compound was also found in the rabbit brain tribulin fraction which selectively inhibits monoamine oxidase A but has no influence on monoamine oxidase B. 4-Hydroxyphenylethanol inhibits monoamine oxidase A in an incompetitive manner with respect to the substrate, serotonin (Ki = 1.4 mM). Possible pathways of 4-hydroxyphenylethanol synthesis and its biological importance as the monoamine oxidase A inhibiting component of tribulin are discussed.

摘要

内源性单胺氧化酶抑制剂刺蒺藜素含有几种可选择性（或非选择性）抑制单胺氧化酶A和B的成分。猪脑刺蒺藜素中选择性抑制单胺氧化酶A的成分经纯化后，利用气相色谱-质谱法鉴定为4-羟基苯乙醇。该化合物也存在于兔脑刺蒺藜素组分中，该组分选择性抑制单胺氧化酶A，但对单胺氧化酶B无影响。4-羟基苯乙醇对底物5-羟色胺以非竞争性方式抑制单胺氧化酶A（Ki = 1.4 mM）。本文讨论了4-羟基苯乙醇的可能合成途径及其作为刺蒺藜素中抑制单胺氧化酶A成分的生物学重要性。