Klein D, Kern R M, Sokol R Z
Department of Endocrinology, Harbor-UCLA Medical Center, Torrance 90502, USA.
Biochem Mol Biol Int. 1995 May;36(1):59-66.
A method is described for quantification of specific proteins after electrophoresis and transfer to immobilization membranes for Western blots. This method is analogous to methods used to correct the amounts of specific transcripts detected on Northern blots. Ponceau S staining of proteins bound to immobilization membranes is efficient and accurate compared to antibody binding in terms of time, effort and cost. Comparison of Ponceau S to other detection and staining methods for quantifying proteins and the basic chemistry of Ponceau S are summarized.
本文描述了一种用于在蛋白质电泳并转移至固定化膜以进行蛋白质免疫印迹后对特定蛋白质进行定量的方法。该方法类似于用于校正Northern印迹上检测到的特定转录本数量的方法。与抗体结合相比，在时间、精力和成本方面，用丽春红S对固定化膜上结合的蛋白质进行染色既高效又准确。总结了丽春红S与其他蛋白质定量检测和染色方法的比较以及丽春红S的基本化学性质。
