Roingeard F, Domenech J, Dayan A, Gihana E, Colombat P, Binet C
Laboratoire d'Hématologie, Hôpital Bretonneau, Tours, France.
Bone Marrow Transplant. 1995 May;15(5):741-7.
We compared the release of granulocyte-macrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor (G-CSF) and tumor necrosis factor alpha (TNF alpha) in the supernatant of long-term bone marrow cultures (LTBMC) derived from 10 control patients and from 14 patients before and 3 months after autologous bone marrow transplantation (BMT). The three cytokines were spontaneously present in the supernatant of cultures established from patients before and after autologous BMT, while GM-CSF remained undetectable in the supernatants of control patients. The maximal levels of cytokines were produced after the first week and were not statistically different between control, patients before and after grafts although the granulocyte-macrophage colony-forming unit (CFU-GM) production in long-term culture (LTC) was lower in patients after graft compared with control patients (median values at LTC initiation: 32 and 158, respectively, P < 0.001 and median values of the total production: 510 and 12406, respectively, P < 0.002). However, GM-CSF was more frequently detected in patients after graft than in control patients. This study demonstrated that the production of GM-CSF, G-CSF and TNF alpha is not impaired in patients after graft (medians 0, 870.5, 173.5 pg/ml and ranges 0-31.2, 0-10 000 and 0-1426, respectively) compared with control patients (medians 0, 69, 66 pg/ml and ranges 0, 0-13 280 and 0-1318, respectively), although patients after graft were shown to have lower marrow CFU-GM counts. These results suggest that the ability of the accessory cells to produce these cytokines was not reduced after autologous BMT.
我们比较了10名对照患者以及14名自体骨髓移植（BMT）患者移植前和移植后3个月的长期骨髓培养物（LTBMC）上清液中粒细胞巨噬细胞集落刺激因子（GM-CSF）、粒细胞集落刺激因子（G-CSF）和肿瘤坏死因子α（TNFα）的释放情况。这三种细胞因子在自体BMT患者移植前和移植后建立的培养物上清液中均自发存在，而在对照患者的上清液中未检测到GM-CSF。细胞因子的最大水平在第一周后产生，对照组、移植前患者和移植后患者之间无统计学差异，尽管移植后患者长期培养（LTC）中的粒细胞巨噬细胞集落形成单位（CFU-GM）产量低于对照患者（LTC开始时的中位数分别为32和158，P<0.001；总产生量的中位数分别为510和12406，P<0.002）。然而，移植后患者中GM-CSF的检测频率高于对照患者。本研究表明，与对照患者（中位数分别为0、69、66 pg/ml，范围分别为0、0-13280、0-1318）相比，移植后患者（中位数分别为0、870.5、173.5 pg/ml，范围分别为0-31.2、0-10000、0-1426）GM-CSF、G-CSF和TNFα的产生未受损，尽管移植后患者的骨髓CFU-GM计数较低。这些结果表明，自体BMT后辅助细胞产生这些细胞因子的能力并未降低。
