Kaplan D, Vazquez M, Lafon S, Schijman A G, Levitus G, Levin M J
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI), Buenos Aires, Argentina.
Biol Res. 1993;26(1-2):273-7.
Molecular expression cloning techniques revealed that patients with the severest clinical form of Chagas disease, chronic Chagas heart disease, presented a strong humoral response against the cloned C-terminal portion of a Trypanosoma cruzi ribosomal P protein. Parasite P antigens identification led to characterize the ribosomal P protein system in T. cruzi. Their exposed location on the ribosome, and the "amplification" of their parasite specific, serine free C-terminal domain, generate a strong parasite specific anti-P response, that in certain cases may induce anti-P autoimmunity.
分子表达克隆技术表明，患有恰加斯病最严重临床形式即慢性恰加斯心脏病的患者，对克氏锥虫核糖体P蛋白的克隆C末端部分呈现出强烈的体液免疫反应。寄生虫P抗原的鉴定有助于对克氏锥虫中的核糖体P蛋白系统进行表征。它们在核糖体上的暴露位置，以及其寄生虫特异性、无丝氨酸C末端结构域的“扩增”，产生了强烈的寄生虫特异性抗P反应，在某些情况下可能会诱导抗P自身免疫。
