Walker J, Watson J, Holmes C, Edelman A, Banting G
Department of Biochemistry, and Biotechnology, University of Bristol, UK.
J Cell Sci. 1995 Jun;108 ( Pt 6):2433-44. doi: 10.1242/jcs.108.6.2433.
We have raised mouse monoclonal antibodies to eight synthetic peptides corresponding to different regions of the human cystic fibrosis transmembrane conductance regulator (CFTR) and rabbit polyclonal antisera to beta-galactosidase fusion proteins which encompass three different regions of CFTR. Immunoblot, immunoprecipitation, immunofluorescence and immunocytochemical experiments demonstrate that, in addition to recognising CFTR, these antibodies recognise one or more immunologically related proteins with a similar molecular mass, calcium responsiveness and tissue distribution to CFTR.
我们已针对与人类囊性纤维化跨膜传导调节因子（CFTR）不同区域相对应的8种合成肽制备了小鼠单克隆抗体，并针对包含CFTR三个不同区域的β-半乳糖苷酶融合蛋白制备了兔多克隆抗血清。免疫印迹、免疫沉淀、免疫荧光和免疫细胞化学实验表明，这些抗体除了识别CFTR外，还识别一种或多种与CFTR具有相似分子量、钙反应性和组织分布的免疫相关蛋白。
