Busch U, Rudolph R
Tierärztlichen Klinik, Freien Universität Berlin.
Tierarztl Prax. 1995 Jun;23(3):280-6.
77 formalin-fixed and paraffin-embedded mammary carcinomas and the regional lymph nodes of bitches were examined by immunocytochemical technique, all of them free of metastases in routine HE-staining. To compare the two methods, two serial sections were cut of three parts of the lymph nodes. One was used for incubation with the antibody AE1, which reacts with cytokeratin subtype I/A, the other for staining with HE. From 60 of the 77 bitches we received information about the operation and the follow up study period (surgical method, new tumours, survival rate and causes of death). These data were compared with the immunocytochemical results of the lymph nodes. In 84.4% of all 77 lymph nodes tumour cell embolism and/or micrometastases were detected. Comparing the two methods, we found that with HE-staining it was only possible to detect two thirds of all micrometastases containing more than 50 tumor cells. Smaller micrometastases were suspicious in a few cases, the majority could not be detected at all. In the follow up study there was evidence of a better prognosis for bitches with tumours detected in an early stage of growth and treated with radical mastectomy. This was independent of a positive or negative result of tumour cells in lymph nodes. The presence of cancer cells in lymph nodes was only important, if either the tumour was treated in an advanced stage or only single complexes or tumour nodes were extirpated. These dogs often showed metastases in the lung.
采用免疫细胞化学技术对77例福尔马林固定、石蜡包埋的母犬乳腺癌及其区域淋巴结进行检查，所有病例在常规苏木精-伊红（HE）染色中均无转移。为比较两种方法，对淋巴结的三个部分制作了两张连续切片。一张用于与抗体AE1孵育，该抗体与细胞角蛋白亚型I/A反应，另一张用于HE染色。我们从77只母犬中的60只获得了手术及随访研究期（手术方法、新肿瘤、生存率和死亡原因）的信息。将这些数据与淋巴结的免疫细胞化学结果进行比较。在所有77个淋巴结中，84.4%检测到肿瘤细胞栓塞和/或微转移。比较这两种方法，我们发现，通过HE染色只能检测到所有含有超过50个肿瘤细胞的微转移的三分之二。较小的微转移在少数情况下可疑，大多数根本无法检测到。在随访研究中，有证据表明，早期生长阶段发现肿瘤并接受根治性乳房切除术的母犬预后较好。这与淋巴结中肿瘤细胞的阳性或阴性结果无关。只有在肿瘤处于晚期治疗或仅切除单个肿瘤复合体或肿瘤结节时，淋巴结中癌细胞的存在才重要。这些犬类经常出现肺部转移。
