Fitzgerald S D, Richard A
Department of Pathology, College of Veterinary Medicine, Michigan State University, Lansing 48909-7576, USA.
Avian Dis. 1995 Apr-Jun;39(2):425-31.
Four different fixatives, A, B, C, and D, were compared for use in routine histology of splenic tissue and for immunohistochemical staining to detect group II avian adenoviral antigen. Sixteen pheasants were experimentally infected with type II adenovirus; spleens from each bird were harvested, divided, and treated with each of the four fixatives. All four fixatives were adequate for routine histology. Fixative B was significantly more sensitive for immunohistochemistry than the three other fixatives, and it also detected the viral antigens at the highest dilutions of the primary antibody.
对四种不同的固定剂A、B、C和D进行了比较，以用于脾脏组织的常规组织学检查以及免疫组织化学染色，以检测II型禽腺病毒抗原。16只雉鸡经实验感染II型腺病毒；从每只鸟身上采集脾脏，进行分割，并用四种固定剂中的每一种进行处理。所有四种固定剂都适用于常规组织学检查。固定剂B在免疫组织化学方面比其他三种固定剂显著更敏感，并且在一抗的最高稀释度下也能检测到病毒抗原。
