Nishioka Y, Dolan B M, Zahed L, Prado V, Tyson H
Department of Biology, McGill University, Montreal, Quebec, Canada.
Mol Biol Evol. 1994 Jan;11(1):146-53. doi: 10.1093/oxfordjournals.molbev.a040083.
A 522-base-long Y-chromosomal sequence was isolated from a BALB/c genomic library and was designated "BF046." It is repeated about 200 times in the male genome, and a difference was detected between the Mus musculus musculus and the M. m. domesticus type Y chromosomes. BF046-related sequences were present over the entire length of the Y chromosome as visualized by in situ hybridization. Southern blot analysis against DNAs isolated from eight species in the genus Mus showed that BF046-related sequences were amplified in the Y chromosomes of three closely related species: M. musculus, M. spicilegus, and M. spretus. To gain insight into the stability of the BF046 sequence family, we isolated 18 additional clones from these three mouse species and compared their sequences. The M. musculus sequences differed from the M. spicilegus and M. spretus sequences by two indels. The remaining parts of the sequences were very similar, but both parsimony and distance-based analytical methods divided the sequences into the same four subgroups, with each species having its own subgroup(s). Thus, the Y chromosomes of M. musculus, M. spicilegus, and M. spretus can be distinguished from one another.
从BALB/c基因组文库中分离出一段长度为522个碱基的Y染色体序列，命名为“BF046”。它在雄性基因组中重复约200次，小家鼠（Mus musculus musculus）和小家鼠驯化亚种（M. m. domesticus）的Y染色体之间存在差异。通过原位杂交观察发现，BF046相关序列存在于Y染色体的全长上。对从鼠属八个物种中分离的DNA进行Southern杂交分析表明，BF046相关序列在三个近缘物种的Y染色体中得到扩增：小家鼠、针毛鼠和斯氏小家鼠。为深入了解BF046序列家族的稳定性，我们从这三个小鼠物种中又分离出18个克隆并比较它们的序列。小家鼠的序列与针毛鼠和斯氏小家鼠的序列有两个插入/缺失差异。序列的其余部分非常相似，但简约法和基于距离的分析方法都将序列分为相同的四个亚组，每个物种都有自己的亚组。因此，小家鼠、针毛鼠和斯氏小家鼠的Y染色体可以相互区分。
