促性腺激素释放激素（GnRH）激动剂可诱导外周血中CD3+CD25+淋巴细胞亚群的下调。

Gonadotropin releasing hormone (GnRH) agonist induces down-regulation of the CD3+CD25+ lymphocyte subpopulation in peripheral blood.

作者信息

Ho H N, Chen H F, Chen S U, Chao K H, Yang Y S, Huang S C, Lee T Y, Gill T J

机构信息

Department of Obstetrics and Gynecology, College of Medicine, Taipei, Taiwan, Republic of China.

出版信息

Am J Reprod Immunol. 1995 Mar;33(3):243-52. doi: 10.1111/j.1600-0897.1995.tb00891.x.

DOI:10.1111/j.1600-0897.1995.tb00891.x

PMID:7546241

Abstract

PROBLEM

To test whether GnRH agonist could alter in vivo human immune cells and whether the alteration is related to the success of pregnancy in an in vitro fertilization-embryo transfer (IVF-ET) program.

METHODS

Thirty-six infertile patients were enrolled under the long protocol of GnRH agonist (buserelin acetate) and superovulation with gonadotropin from our IVF-ET program. Peripheral B cells, NK cells, CD4+ and CD8+ T cells, and the expression of CD69, CD25, HLA-DR, and CD71 antigens on the T cells were serially examined by dual-color flow cytometry.

RESULTS

B cells, NK cells, CD8+ T cells, and CD71+ T lymphocyte subpopulations were not changed throughout the whole course of treatment. CD4+ T cell and CD25+ T cell subpopulations were significantly down-regulated when the GnRH agonist was used for approximately 2 wk. CD3+CD69+, CD3+CD25+, and CD3+DR+ lymphocyte subpopulations were increased at 7 days (during implantation) and at 14 days after embryo transfer in pregnant patients, but not in patients who failed to get pregnant.

CONCLUSIONS

The GnRH agonist had a transiently immunosuppressive effect on CD4+ and CD25+ T cells, but CD69+, CD25+, and HLA-DR+ T cells were activated during and after successful implantation.

摘要

问题

检测促性腺激素释放激素（GnRH）激动剂是否能改变体内人类免疫细胞，以及这种改变是否与体外受精-胚胎移植（IVF-ET）项目中的妊娠成功相关。

方法

从我们的IVF-ET项目中选取36例采用GnRH激动剂（醋酸布舍瑞林）长方案并使用促性腺激素进行超排卵的不孕患者。通过双色流式细胞术连续检测外周血B细胞、自然杀伤（NK）细胞、CD4⁺和CD8⁺ T细胞，以及T细胞上CD69、CD25、人类白细胞抗原-DR（HLA-DR）和CD71抗原的表达。

结果

在整个治疗过程中，B细胞、NK细胞、CD8⁺ T细胞和CD71⁺ T淋巴细胞亚群未发生变化。当使用GnRH激动剂约2周时，CD4⁺ T细胞和CD25⁺ T细胞亚群显著下调。在胚胎移植后7天（着床期）和14天时，妊娠患者的CD3⁺CD69⁺、CD3⁺CD25⁺和CD3⁺DR⁺淋巴细胞亚群增加，而未妊娠患者则未增加。