Shangary S, Singh J, Kamboj S S, Kamboj K K, Sandhu R S
Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar, India.
Phytochemistry. 1995 Sep;40(2):449-55. doi: 10.1016/0031-9422(95)00229-z.
Four new monocot lectins from the tubers of araceous plants, namely, Arisaema consanguineum Schott (ACA), A. curvatum Kunth (ACmA) and Sauromatum guttatum Schott (SGA) from the tribe Areae, and Gonatanthus pumilus D. Don (GPA) from the tribe Colocasieae have been purified by affinity chromatography on asialofetuin-linked amino activated silica beads. These lectins possess similar physicochemical and biological properties. All the lectins gave a single peak on HPLC size exclusion and cation exchange columns, and a single band on PAGE, (pH 4.5). In SDS-PAGE, all the lectins gave a single band corresponding to a subunit of M(r) 1,3000. All the lectins yielded multiple peaks on anion-exchange column, multiple bands on non-denatured PAGE (pH 8.3) and a family of bands on isoelectric focusing. The lectins agglutinate rabbit, rat and sheep red blood cells (RBCs) but are inactive towards human ABO erythrocytes. The haemagglutination activity of these lectins is inhibited by asialofetuin only, while simple sugars/derivatives including chitin, porcine mucin and fetuin did not react. In serological studies against rabbit anti-SGA serum, all four lectins produced immunoprecipitin lines. The lectins within each tribe were identical but the lectins belonging to the tribe Areae were only partially identical to the lectins from the tribe Colocasieae.
从天南星科植物块茎中分离得到了四种新的单子叶植物凝集素，分别是天南星族的天南星（ACA）、弯苞南星（ACmA）和疣柄魔芋（SGA），以及芋族的矮小曲苞芋（GPA），它们通过在去唾液酸胎球蛋白连接的氨基活化硅胶珠上进行亲和层析得以纯化。这些凝集素具有相似的物理化学和生物学特性。所有凝集素在高效液相色谱尺寸排阻柱和阳离子交换柱上均给出单一峰，在聚丙烯酰胺凝胶电泳（pH 4.5）上呈现单一谱带。在十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳中，所有凝集素均给出对应于分子量为13000亚基的单一谱带。所有凝集素在阴离子交换柱上产生多个峰，在非变性聚丙烯酰胺凝胶电泳（pH 8.3）上出现多个谱带，在等电聚焦时呈现一系列谱带。这些凝集素能凝集兔、大鼠和绵羊的红细胞，但对人ABO血型红细胞无活性。这些凝集素的血凝活性仅被去唾液酸胎球蛋白抑制，而包括几丁质、猪粘蛋白和胎球蛋白在内的单糖/衍生物则无反应。在针对兔抗SGA血清的血清学研究中，所有四种凝集素均产生免疫沉淀线。每个族内的凝集素是相同的，但天南星族的凝集素与芋族的凝集素仅部分相同。
