Murphy N R, Leinbach S S, Hellwig R J
Biotechniques. 1995 Jun;18(6):1068-73.
The performance characteristics of a potent new RNase inhibitor have been evaluated, as have applications for its use in molecular biology. PRIME Inhibitor is a protein, not related to the commonly available human placental RNase inhibitors (HPRI). It has a high specific activity, enhanced temperature stability, broad reaction pH range and significantly greater cost-effectiveness than commercial HPRI. PRIME Inhibitor is suitable for use in in vitro transcription, in vitro translation, first- and second-strand cDNA synthesis, preparation of RNA and mRNA, and reverse transcription-polymerase chain reaction.
一种强效新型核糖核酸酶抑制剂的性能特征已得到评估，其在分子生物学中的应用也已得到评估。PRIME抑制剂是一种蛋白质，与常用的人胎盘核糖核酸酶抑制剂（HPRI）无关。它具有高比活性、增强的热稳定性、较宽的反应pH范围，且成本效益比市售的HPRI显著更高。PRIME抑制剂适用于体外转录、体外翻译、第一链和第二链cDNA合成、RNA和mRNA制备以及逆转录-聚合酶链反应。
