Edward M
University of Glasgow, Department of Dermatology, U.K.
Br J Dermatol. 1995 Aug;133(2):223-30. doi: 10.1111/j.1365-2133.1995.tb02619.x.
Fibroblasts grown within contracted collagen lattices synthesize substantially less glycosaminoglycans than fibroblasts grown as monolayers on a plastic substrate. [3H]glucosamine incorporation into hyaluronate was reduced by 70%, and incorporation into sulphated glycosaminoglycans was reduced by 40%. However, incorporation into heparan sulphate and chondroitin sulphates was reduced by 14 and 49%, respectively, resulting in a substantial change in the proportions of the individual glycosaminoglycans. On the basis of [3H]glucosamine incorporation, hyaluronate constituted 80% of the total glycosaminoglycans synthesized in monolayer cultures, but only 67% in collagen lattice cultures. Incorporation of 35SO4 into chondroitin sulphates was reduced by 22%, whereas no change was observed in heparan sulphates following culture within collagen lattices. Exposure of the fibroblast cultures to retinoic acid (10(-6) mol/l) and retinyl propionate (2 x 10(-6) mol/l) resulted in a decrease in the incorporation of [3H]glucosamine into hyaluronate by up to 41% in monolayer cultures, and 25% in collagen lattice cultures. The retinoids stimulated the incorporation of [3H]glucosamine into heparan sulphate by up to 72%, and chondroitin sulphates by up to 30%, whereas 35SO4 incorporation remained essentially unaltered. Only modest changes in the incorporation of both isotopes into fibroblast sulphated glycosaminoglycans were observed following exposure to the retinoids in lattice cultures. Q-Sepharose ion-exchange chromatography at pH 2.0 revealed that there was no change in the degree of polymer sulphation of either chondroitin sulphate or heparan sulphate isolated from collagen lattice cultures compared with monolayer cultures. Retinoic acid (10(-6) mol/l) treatment did, however, reduce the degree of polymer sulphation of heparan sulphates and chondroitin sulphates in both monolayer and lattice cultures.(ABSTRACT TRUNCATED AT 250 WORDS)
在收缩的胶原晶格中生长的成纤维细胞合成的糖胺聚糖比在塑料基质上单层生长的成纤维细胞少得多。[3H]葡萄糖胺掺入透明质酸减少了70%，掺入硫酸化糖胺聚糖减少了40%。然而，掺入硫酸乙酰肝素和硫酸软骨素分别减少了14%和49%，导致单个糖胺聚糖的比例发生了显著变化。基于[3H]葡萄糖胺掺入量，透明质酸在单层培养物中合成的总糖胺聚糖中占80%，但在胶原晶格培养物中仅占67%。35SO4掺入硫酸软骨素减少了22%，而在胶原晶格中培养后硫酸乙酰肝素未观察到变化。将成纤维细胞培养物暴露于视黄酸（10(-6)mol/L）和视黄醇丙酸酯（2×10(-6)mol/L）导致单层培养物中[3H]葡萄糖胺掺入透明质酸减少高达41%，胶原晶格培养物中减少25%。类视黄醇刺激[3H]葡萄糖胺掺入硫酸乙酰肝素高达72%，掺入硫酸软骨素高达30%，而35SO4掺入基本不变。在晶格培养物中暴露于类视黄醇后，观察到两种同位素掺入成纤维细胞硫酸化糖胺聚糖的变化不大。在pH 2.0条件下的Q-琼脂糖离子交换色谱显示，与单层培养物相比，从胶原晶格培养物中分离的硫酸软骨素或硫酸乙酰肝素的聚合物硫酸化程度没有变化。然而，视黄酸（10(-6)mol/L）处理确实降低了单层和晶格培养物中硫酸乙酰肝素和硫酸软骨素的聚合物硫酸化程度。（摘要截短至250字）
