Okamoto M, Arakawa K, Fushiki S
Second Department of Surgery, Kyoto Prefectural University of Medicine, Japan.
Int J Exp Pathol. 1995 Aug;76(4):277-86.
Rat small bowel transplantation was performed in three groups: syngeneic graft, Lewis to Lewis; allogeneic graft, Brown Norway to Lewis; and allogeneic graft with the immunosuppressant FK506 (1 mg/kg). Standard histology and bromodeoxyuridine (BrdU) labelling immunohistochemistry were applied to evaluate the in situ spontaneous proliferating status of the mononuclear cells in the graft lymphoid tissue. In untreated allograft, BrdU-labelling index (BLI) in lamina propria lymphocytes increased significantly on the 5th post-operative day (POD), when no sign of rejection was detected in the villous architecture, compared with isograft (11.8 +/- 2.0% vs 1.9 +/- 0.5%, P < 0.05). Changes in the BLI of lamina propria lymphocyte were also detected on the 3rd and 7th POD, although there were statistically less significant than on the 5th POD. In intraepithelial lymphocytes the changes in BLI were more probably caused by ischaemic damage than immunological reaction. In Peyer's patches and mesenteric lymph nodes, elevation of BLI was specific to rejection like that seen in lamina propria lymphocytes. By administration of FK506, both the elevation of BLI and the histopathological changes were suppressed successfully except for a transient elevation of BLI in Peyer's patches and mesenteric lymph nodes on the 3rd POD.
同基因移植，即Lewis大鼠之间的移植；异基因移植，即Brown Norway大鼠到Lewis大鼠的移植；以及使用免疫抑制剂FK506（1毫克/千克）的异基因移植。应用标准组织学和溴脱氧尿苷（BrdU）标记免疫组织化学来评估移植淋巴组织中单核细胞的原位自发增殖状态。在未处理的同种异体移植中，与同基因移植相比，术后第5天固有层淋巴细胞的BrdU标记指数（BLI）显著增加（11.8±2.0%对1.9±0.5%，P<0.05），此时在绒毛结构中未检测到排斥迹象。在术后第3天和第7天也检测到了固有层淋巴细胞BLI的变化，尽管在统计学上不如第5天显著。在上皮内淋巴细胞中，BLI的变化更可能是由缺血损伤而非免疫反应引起的。在派尔集合淋巴结和肠系膜淋巴结中，BLI的升高与固有层淋巴细胞中所见的排斥反应一样具有特异性。通过给予FK506，除了在术后第3天派尔集合淋巴结和肠系膜淋巴结中BLI有短暂升高外，BLI的升高和组织病理学变化均得到成功抑制。
