Fujita T, Mandel J L, Shirasawa T, Hino O, Shirai T, Maruyama N
Department of Molecular Biology, Tokyo Metropolitan Institute of Gerontology, Japan.
Biochim Biophys Acta. 1995 Sep 19;1263(3):249-52. doi: 10.1016/0167-4781(95)00120-6.
We have isolated and characterized a cDNA clone encoding human homologue of senescence marker protein-30 (SMP30), a calcium binding protein also called regucalcin (RC). This clone (pHSMP6) has 1356 base pairs (bp) and contains an open reading frame of 897 bp, which encodes 299 amino acids. The estimated molecular weight of the deduced polypeptide is 33,250 and pI is 5.836. The homology of amino acid sequences between human homologue and rat SMP30 is 88.6%. Using pHSMP6 as a probe, the chromosomal location of the human homologue of SMP30 gene was determined. The results of regional mapping using a panel of 11 rodent-human somatic hybrids indicated that the gene is located in the p11.3-q11.2 segment of the X chromosome. This gene thus could be a candidate for one of the X-linked diseases mapped to this regions.
我们已经分离并鉴定了一个编码衰老标记蛋白30（SMP30）人类同源物的cDNA克隆，SMP30是一种钙结合蛋白，也被称为调钙蛋白（RC）。该克隆（pHSMP6）有1356个碱基对（bp），包含一个897 bp的开放阅读框，编码299个氨基酸。推导的多肽估计分子量为33,250，等电点为5.836。人类同源物与大鼠SMP30的氨基酸序列同源性为88.6%。使用pHSMP6作为探针，确定了SMP30基因人类同源物的染色体定位。使用一组11个啮齿动物-人类体细胞杂种进行区域定位的结果表明，该基因位于X染色体的p11.3-q11.2区段。因此，该基因可能是定位到该区域的X连锁疾病之一的候选基因。
