牛精液纤溶酶基因的启动子分析

Promoter analysis of the bovine gene for seminalplasmin.

作者信息

Herbers J, Scheit K H

机构信息

Max-Planck-Institute for Biophysical Chemistry, Department of Molecular Biology, Göttingen, Germany.

出版信息

Biochim Biophys Acta. 1995 Sep 19;1263(3):271-4. doi: 10.1016/0167-4781(95)00139-8.

DOI:10.1016/0167-4781(95)00139-8

PMID:7548218

Abstract

In this study we mapped the transcriptional initiation site of the gene for seminalplasmin (SAP) by primer extension analysis, situated 125 nucleotides upstream of the translational initiation site of the SAP-specific mRNA. We showed that the TATA-box in position -30 of the SAP gene is part of a functional promoter. A 280 bp region of the 5'-flanking region exerted a strong positive effect on promoter activity. In this region we identified consensus sequences for the transcriptional control elements AP1, AP2, PEA3 and GATA.

摘要

在本研究中，我们通过引物延伸分析确定了精浆酶（SAP）基因的转录起始位点，该位点位于SAP特异性mRNA翻译起始位点上游125个核苷酸处。我们发现，SAP基因-30位的TATA框是功能性启动子的一部分。5'侧翼区的一个280 bp区域对启动子活性有很强的正向作用。在该区域，我们鉴定出了转录控制元件AP1、AP2、PEA3和GATA的共有序列。