通过棒状杆菌16S rRNA基因的比较序列分析确定幼鼠尿路结石的细菌病原体

Assignment of the bacterial agent of urinary calculus in young rats by the comparative sequence analysis of the 16S rRNA genes of corynebacteria.

作者信息

Takahashi T, Tsuji M, Kikuchi N, Ishihara C, Osanai T, Kasai N, Yanagawa R, Hiramune T

出版信息

J Vet Med Sci. 1995 Jun;57(3):515-7. doi: 10.1292/jvms.57.515.

DOI:10.1292/jvms.57.515

Abstract

Comparative 16S rRNA gene sequencing was used to assign four isolates of spontaneous urinary calculus in young laboratory rats. The phylogenetic relationships among the rat isolates and selected species of corynebacteria were also inferred. Based on the homology and evolutionary distance analysis, the 16S rRNA genes of the rat isolates were almost identical with that of Corynebacterium renale ATCC 19412. Also the results of the phylogenetic analysis showed a close relationship among the isolates and C. renale, but they were clearly different from C. pilosum, C. cystitidis, C. kutscheri and Rhodococcus equi. The results of the present study and previously published biochemical data demonstrate that the organism involving urinary infections in young rats is identified to be C. renale.

摘要

采用比较16S rRNA基因测序法对4株年轻实验大鼠自发性尿路结石分离株进行分类。同时还推断了大鼠分离株与所选棒状杆菌属物种之间的系统发育关系。基于同源性和进化距离分析，大鼠分离株的16S rRNA基因与肾棒状杆菌ATCC 19412的16S rRNA基因几乎相同。系统发育分析结果也表明，分离株与肾棒状杆菌关系密切，但与毛状棒状杆菌、膀胱炎棒状杆菌、库氏棒状杆菌和马红球菌明显不同。本研究结果和先前发表的生化数据表明，导致幼鼠尿路感染的病原体为肾棒状杆菌。