Sato K, Tokuhisa S, Inaba Y
Kyushu Branch Laboratory, National Institute of Animal Health, Kagoshima, Japan.
J Vet Med Sci. 1995 Jun;57(3):569-70. doi: 10.1292/jvms.57.569.
The growth of group A human, bovine, equine and porcine rotaviruses were enhanced by pretreatment of virus with pancreatin, trypsin, protease, alkaline phosphatase or pepsin and incorporation of these enzymes in maintenance medium. In contrast, alpha-amylase or lipase inhibited the growth of equine and porcine rotaviruses. The other enzymes, adenosine deaminase, lactase, lysozyme, ribonuclease or triose-phosphate isomerase gave little or no change in the growth of all four rotaviruses.
用胰蛋白酶、胰凝乳蛋白酶、蛋白酶、碱性磷酸酶或胃蛋白酶预处理病毒，并将这些酶加入维持培养基中，可增强A组人、牛、马和猪轮状病毒的生长。相比之下，α-淀粉酶或脂肪酶可抑制马和猪轮状病毒的生长。其他酶，如腺苷脱氨酶、乳糖酶、溶菌酶、核糖核酸酶或磷酸丙糖异构酶，对所有四种轮状病毒的生长几乎没有影响或没有影响。
