Mutasa H C
Department of Medical Laboratory Technology, University of Zimbabwe Medical School, Avondale, Harare.
Biotech Histochem. 1995 May;70(3):124-34. doi: 10.3109/10520299509108329.
Granule protein deficiencies in morphologically mature neutrophil cells of peripheral blood from human patients with acute myeloid leukemia was demonstrated using post-embedding immunocytochemistry. Abnormal immunoreactivity of granule proteins was detected in seven of nine patients. Decreased immunoreactivity patterns were found more for the primary granule markers elastase and myeloperoxidase than for the secondary granule marker lactoferrin. Leukemias with a predominant myeloid component, in contrast to those with a predominant monocytoid component, had more neutrophil cells showing immunodeficiencies for one or more granule markers. The proportion of neutrophil cells showing immunodeficiencies varied greatly for each granule marker; more variation was obtained for elastase, lactoferrin and myeloperoxidase than for lysozyme, possibly because lysozyme is a marker for both granule types. In addition, no correlation could be found between any of the immunoreactivity deficiencies for the neutrophil granule glycoproteins elastase, lactoferrin, lysozyme and myeloperoxidase and the abundance of a particular set of ultrastructural features in the circulating leukemic cells from any of the nine patients. Nonetheless, most of the immature myeloid cells from peripheral blood of leukemic patients showing neutrophil protein immunoreactivity abnormalities in one or more granule markers often and randomly displayed one or more unusual ultrastructural features. The clinical and pathological significance of neutrophil granule protein deficiencies and the abundance of fibrillar structures in malignant myeloid cells presently is uncertain.
采用包埋后免疫细胞化学方法，证实了急性髓系白血病患者外周血形态学成熟中性粒细胞中的颗粒蛋白缺乏。在9例患者中的7例检测到颗粒蛋白的异常免疫反应性。与二级颗粒标志物乳铁蛋白相比，一级颗粒标志物弹性蛋白酶和髓过氧化物酶的免疫反应性降低模式更为常见。与主要为单核细胞成分的白血病相比，主要为髓系成分的白血病有更多的中性粒细胞对一种或多种颗粒标志物表现出免疫缺陷。显示免疫缺陷的中性粒细胞比例因每种颗粒标志物而异；弹性蛋白酶、乳铁蛋白和髓过氧化物酶的变异比溶菌酶更多，这可能是因为溶菌酶是两种颗粒类型的标志物。此外，在9例患者中，中性粒细胞颗粒糖蛋白弹性蛋白酶、乳铁蛋白、溶菌酶和髓过氧化物酶的任何免疫反应性缺陷与循环白血病细胞中一组特定超微结构特征的丰度之间均未发现相关性。尽管如此，白血病患者外周血中大多数在一种或多种颗粒标志物上显示中性粒细胞蛋白免疫反应性异常的未成熟髓系细胞经常且随机地表现出一种或多种异常超微结构特征。目前，中性粒细胞颗粒蛋白缺乏和恶性髓系细胞中纤维状结构丰度的临床和病理意义尚不确定。
