Romero A, Gómez F, Villamayor F, Sacristán A, Ortíz J A
Departamento de Toxicología, Centro de Investigación del Grupo Ferrer, Barcelona, Spain.
Cell Prolif. 1995 Jul;28(7):393-401. doi: 10.1111/j.1365-2184.1995.tb00080.x.
Two groups of male rats were treated orally for 60 days with ebrotidine and cimetidine, used as reference standard, respectively. The dose used of both drugs was 500 mg/kg. A third group, used as control, received 10 ml/kg of an aqueous agar suspension. After receiving the last dose, the animals were killed by inhalation of CO2 in a sealed chamber and the stomachs were removed for histological preparation. The purpose of this study was to count antral G-cells throughout the gastric mucosa by light microscope. A PAP system-associated antigastrin immunohistochemical method was used for cell identification. Cell density, with respect to their location in the gastric mucosa and the treatments given, was calculated using image analysis techniques. The results showed that ebrotidine did not cause any significant differences in the density of the population of these cells compared with the control group, while cimetidine induced a significant proliferation of antral G-cells.
将两组雄性大鼠分别口服埃布罗替丁和作为参考标准的西咪替丁，持续60天。两种药物的使用剂量均为500毫克/千克。第三组作为对照组，接受10毫升/千克的琼脂水悬浮液。在接受最后一剂后，通过在密封舱中吸入二氧化碳处死动物，并取出胃进行组织学制备。本研究的目的是通过光学显微镜对整个胃黏膜中的胃窦G细胞进行计数。使用与PAP系统相关的抗胃泌素免疫组织化学方法进行细胞鉴定。使用图像分析技术计算细胞密度，该密度与它们在胃黏膜中的位置以及所给予的治疗有关。结果表明，与对照组相比，埃布罗替丁在这些细胞群体的密度上未引起任何显著差异，而西咪替丁则诱导胃窦G细胞显著增殖。
