Varga J M, Kalchschmid G, Bellon B, Kuhn J, Druet P, Fritsch P
Department of Dermatology, University of Innsbruck, Austria.
Int Arch Allergy Immunol. 1995 Oct;108(2):196-9. doi: 10.1159/000237139.
The incidence of cross-stimulations by natural allergens was investigated using RBL-2H3 cells sensitized with five different mouse monoclonal anti-DNP IgEs and four mercury-induced rat monoclonal IgEs. Cells sensitized with 3 of the 5 monoclonal anti-DNP IgEs (clones SPE-7, SRT-1, LB4) responded by serotonin release upon stimulation by natural allergens such as Dermatophagoides pteronyssinus, horse dander and mugwort extracts. Serotonin release could be inhibited by monovalent DNP-lysine, indicating the involvement of DNP-binding sites of IgEs. Two of the clones (LO-DNP-30 and LA2) were negative on all tests with allergens. All but one (Hg32) of the mercury-induced rat IgE monoclonal antibodies tested positive with DNP-BSA, and with at least one of the six allergen extracts. IgE clone Hg12 mediated serotonin release with 5 of the 6 allergens tested.
使用用五种不同的小鼠单克隆抗 DNP IgE 和四种汞诱导的大鼠单克隆 IgE 致敏的 RBL-2H3 细胞，研究了天然过敏原交叉刺激的发生率。用 5 种单克隆抗 DNP IgE 中的 3 种（克隆 SPE-7、SRT-1、LB4）致敏的细胞在受到天然过敏原如屋尘螨、马皮屑和艾蒿提取物刺激后，通过血清素释放作出反应。单价 DNP-赖氨酸可抑制血清素释放，表明 IgE 的 DNP 结合位点参与其中。其中两个克隆（LO-DNP-30 和 LA2）在所有过敏原测试中均为阴性。除一种（Hg32）外，所有测试的汞诱导大鼠 IgE 单克隆抗体与 DNP-BSA 以及六种过敏原提取物中的至少一种呈阳性反应。IgE 克隆 Hg12 在测试的 6 种过敏原中的 5 种中介导了血清素释放。
