D'Aoust J Y, Sewell A M, McDonald C
Health Canada, Food Directorate, Sir F.G. Banting Research Centre, Ottawa, ON.
J AOAC Int. 1995 Sep-Oct;78(5):1322-7.
Refrigerated preenrichment 72 h and selective enrichment cultures arising from 25 g analytical units of dry foods can be used to increase the analytical flexibility and productivity of laboratories for the detection of foodborne Salmonella spp. by AOAC method 994.04. Results of this intralaboratory study using artificially contaminated dry foods validate the extended application of the refrigerated preenrichment approach to dry food composites (375 g). All samples found to be contaminated by AOAC/Bacteriological Analytical Manual methods were identified readily from the homologous, refrigerated preenrichment broth cultures. This extended application of the refrigeration approach was recently adopted First Action by AOAC and was included as a modification to method 994.04. In addition, ancillary work on the diagnostic value of prolonged (48 h) incubation of lysine iron (LI) agar as described in the AOAC Official Method 967.26 led to a recommendation that the 48 h period of incubation be revoked in favor of a 24 h incubation of inoculated LI medium.
通过AOAC方法994.04，对25克分析单位的干燥食品进行72小时的冷藏预富集以及选择性富集培养，可用于提高实验室检测食源沙门氏菌的分析灵活性和效率。这项使用人工污染干燥食品的实验室内部研究结果，验证了冷藏预富集方法在干燥食品混合物（375克）中的扩展应用。所有通过AOAC/《细菌学分析手册》方法检测出受污染的样品，都能从同源的冷藏预富集肉汤培养物中轻松鉴定出来。这种冷藏方法的扩展应用最近被AOAC首次采用，并作为对方法994.04的修改内容纳入其中。此外，关于AOAC官方方法967.26中所述赖氨酸铁（LI）琼脂延长（48小时）培养的诊断价值的辅助工作，导致一项建议，即取消48小时的培养时间，转而采用接种LI培养基24小时的培养时间。
