利用抗胰岛素样生长因子-I（IGF-I）单克隆抗体进行胰岛素样生长因子-I（IGF-I）放射免疫测定的研究。

Studies of radioimmunoassay for measurement of insulin-like growth factor-I (IGF-I) by using anti-IGF-I monoclonal antibody.

作者信息

Tominaga M, Tominaga K, Kinoshita Y, Terano Y, Yamamoto K

机构信息

Department of Physiology, Osaka City University Medical School, Japan.

出版信息

Cell Mol Biol (Noisy-le-grand). 1995 Jun;41(4):505-14.

PMID:7549787

Abstract

Radioimmunoassay system for measurement of insulin-like growth factor-I (IGF-I) was established by using anti-recombinant human IGF-I monoclonal antibody (MAb). This MAb is capable of recognizing not only human but also rat IGF-I. It was thus suggested that this RIA system can quantify IGF-I in human and rat sera. MAb used in this paper was clarified to be an antibody against the common epitope of C-region of human and rat IGF-I. Gly32-Ser33-Ser34 sequence of C-region of IGF-I is discussed to be an antigenic determinant to which this antibody might specifically bind. MAb does not cross-react with proinsulin and insulin as well as anti-IGF-I polyclonal antibody (PAb). And it is general that PAb has almost 2% cross-reactivity with IGF-II. But this MAb did not cross-react with IGF-II. Actually, the value of IGF-I measured by this system was lower than that measured by RIA using PAb.

摘要

利用抗重组人胰岛素样生长因子-I（IGF-I）单克隆抗体（MAb）建立了用于测量胰岛素样生长因子-I（IGF-I）的放射免疫分析系统。该单克隆抗体不仅能够识别人类IGF-I，还能识别大鼠IGF-I。因此表明该放射免疫分析系统能够定量检测人和大鼠血清中的IGF-I。本文中使用的单克隆抗体被确认为是针对人和大鼠IGF-I C区域共同表位的抗体。IGF-I C区域的Gly32-Ser33-Ser34序列被认为是该抗体可能特异性结合的抗原决定簇。该单克隆抗体与胰岛素原、胰岛素以及抗IGF-I多克隆抗体（PAb）均无交叉反应。一般来说，多克隆抗体与IGF-II有近2%的交叉反应。但该单克隆抗体与IGF-II无交叉反应。实际上，用该系统测得的IGF-I值低于使用多克隆抗体的放射免疫分析测得的值。