Ryan D H, Tang J
Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, New York 14642, USA.
Leuk Lymphoma. 1995 May;17(5-6):375-89. doi: 10.3109/10428199509056848.
Recent advances in the ability to culture normal human B cell precursors have emphasized the supportive relationship between these cells and stromal cells in the bone marrow microenvironment. It is now possible to examine the role of adhesion molecules and cytokines in the regulation of different stages of human lymphopoiesis using these culture systems. Direct cell-cell adhesion mediated by the integrin adhesion molecule VLA-4 plays a critical role in supporting stromal dependent proliferation of human B cell precursors. In addition, human B precursor cell lines migrate underneath the stromal layer. This transmigration is VLA-4 dependent but not inhibitable by antibody to known VLA-4 ligands. IL-7 is secreted by the stromal layer, and is necessary for stromal-dependent proliferation of early human B cell precursors. Proliferation of early human B cell precursors or mature B cells. Since the bone marrow stroma is a source of cytokines with B cell precursor growth stimulatory activity, it is possible that adhesion interactions may play a co-stimulatory role with respect to cytokine secretion or response. As the cytokine requirements for human B cell lymphopoiesis become more completely defined, it will be important to uncover the cell-cell signals that regulate lymphopoiesis either directly or through modulation of cytokine secretion by supporting cells in the bone marrow microenvironment. The dependent relationship between human B cell precursors and the bone marrow microenvironment provides a model system for these cell-cell interactions which may be applicable to progenitor development in other lineages.
正常人类B细胞前体培养能力的最新进展强调了这些细胞与骨髓微环境中基质细胞之间的支持关系。现在可以利用这些培养系统来研究黏附分子和细胞因子在人类淋巴细胞生成不同阶段调节中的作用。由整合素黏附分子VLA-4介导的直接细胞间黏附在支持人类B细胞前体的基质依赖性增殖中起关键作用。此外，人类B前体细胞系在基质层下方迁移。这种迁移依赖于VLA-4，但不受已知VLA-4配体抗体的抑制。IL-7由基质层分泌，是早期人类B细胞前体基质依赖性增殖所必需的。早期人类B细胞前体或成熟B细胞的增殖。由于骨髓基质是具有B细胞前体生长刺激活性的细胞因子的来源，黏附相互作用可能在细胞因子分泌或反应方面发挥共刺激作用。随着对人类B细胞淋巴细胞生成的细胞因子需求得到更全面的定义，揭示直接调节淋巴细胞生成或通过调节骨髓微环境中支持细胞的细胞因子分泌来调节淋巴细胞生成的细胞间信号将变得很重要。人类B细胞前体与骨髓微环境之间的依赖关系为这些细胞间相互作用提供了一个模型系统，这可能适用于其他谱系的祖细胞发育。
