Pyne C K, Loones M T, Simon F, Zhou Z J
CNRS-URA 1135, Laboratoire de Génétique du Développement, Université P-et-M-Curie, Paris, France.
Biol Cell. 1995;83(2-3):191-200. doi: 10.1016/0248-4900(96)81308-6.
The lampbrush chromosomes of the urodele Pleurodeles waltl have been studied using the mitosis-specific monoclonal antibody MPM-2. Immunofluorescence studies revealed that MPM-2 stains structures associated with axial granules, numerous other chromomeres, telomeres and certain chiasmata. These structures showed a negative reaction with the anti-DNA monoclonal antibody AC-30-10. In course of meiotic condensation of the chromosomes, in growing and maturating oocytes, the number of such structures associated with the chromosome axis was found to diminish progressively. These granular structures have been found to be formed by fine fibrils about 5 nm in diameter. Immunogold labeling confirmed the results of immunofluorescence studies. MPM-2 was also found to stain two other types of structures observed in association with the lampbrush chromosome axis in P waltl, viz the sphere organelle (only in later stages of oogenesis) and the structure known as 'M' which is singular to this material.
已使用有丝分裂特异性单克隆抗体MPM-2对有尾目动物肋突螈的灯刷染色体进行了研究。免疫荧光研究表明，MPM-2可对与轴粒、许多其他染色粒、端粒和某些交叉相关的结构进行染色。这些结构与抗DNA单克隆抗体AC-30-10呈阴性反应。在卵母细胞生长和成熟过程中，随着染色体减数分裂浓缩过程的进行，发现与染色体轴相关的此类结构的数量逐渐减少。已发现这些颗粒结构由直径约5纳米的细纤维形成。免疫金标记证实了免疫荧光研究的结果。还发现MPM-2可对在肋突螈灯刷染色体轴上观察到的另外两种结构进行染色，即球形细胞器（仅在卵子发生后期）和该材料特有的称为“M”的结构。
