Terpstra A H, von Duvillard S P
Brown University Program-in-Medicine, Miriam Hospital, Providence RI 02906, USA.
Biochem Mol Biol Int. 1995 Apr;35(5):937-47.
We studied the effect of temperature, concentration of low-density (LDL) and high-density lipoprotein (HDL) cholesteryl esters, and the concentration of human lipoprotein deficient serum (LPDS, a source of cholesteryl ester transfer activity) on the fluxes of cholesteryl esters between human LDL and HDL in vitro. Incubation temperatures above 37 degrees C resulted in an increase of the fluxes, and a six-fold increase was observed at 55 degrees C. Decreasing the temperature had the opposite effect, and no transfer activity was measured below 10 degrees C. Increasing the concentration of LPDS without changing the concentration of LDL and HDL resulted in a linear increase of the fluxes. Increasing the concentration of LDL and HDL cholesteryl esters without changing the concentration of LPDS resulted in a nonlinear increase of the fluxes, and the results could be described by a Lineweaver-Burk plot. When both the concentration of LPDS and HDL and LDL cholesteryl esters were increased in the same proportions, the fluxes increased more than proportionally.
我们在体外研究了温度、低密度（LDL）和高密度脂蛋白（HDL）胆固醇酯的浓度以及人脂蛋白缺乏血清（LPDS，胆固醇酯转移活性的来源）的浓度对人LDL和HDL之间胆固醇酯通量的影响。高于37℃的孵育温度导致通量增加，在55℃时观察到通量增加了六倍。降低温度则产生相反的效果，在10℃以下未检测到转移活性。在不改变LDL和HDL浓度的情况下增加LPDS的浓度导致通量呈线性增加。在不改变LPDS浓度的情况下增加LDL和HDL胆固醇酯的浓度导致通量呈非线性增加，结果可用Lineweaver-Burk图描述。当LPDS以及HDL和LDL胆固醇酯的浓度以相同比例增加时，通量增加超过比例。
