Moraes M S, Leite S R
Instituto de Química, Universidade Estadual Paulista, Araraquara, SP.
Braz J Med Biol Res. 1994 Nov;27(11):2635-8.
We report the in vitro inhibitory effect of very low concentrations of aluminum salts (IC50 = 4.1 x 10(-12 M) on bovine brain acetylcholinesterase (AChE). The enzymatic assays were performed using acetylcholine bromide in a buffered pH 7.4 solution at 37 degrees C. The relevant enzyme interacting species is the Al3+ ion, whose concentrations were fixed at pM levels by a citrate metal ion buffer system. The IC50 demonstrates that Al3+ is a potent inhibitor of AChE.
我们报告了极低浓度铝盐（IC50 = 4.1×10⁻¹² M）对牛脑乙酰胆碱酯酶（AChE）的体外抑制作用。酶活性测定是在37℃、pH 7.4的缓冲溶液中使用溴化乙酰胆碱进行的。相关的酶相互作用物种是Al³⁺离子，其浓度通过柠檬酸盐金属离子缓冲系统固定在皮摩尔水平。IC50表明Al³⁺是AChE的有效抑制剂。
