Héron A, Borghi H, Callé A, Bourguignon J, Diarra-Mehrpour M, Martin J P, Sesboüé R
INSERM Unité 295, Faculté de Médecine-Pharmacie de Rouen, St Etienne Rouvray, France.
Cell Biol Int. 1995 Jul;19(7):593-602. doi: 10.1006/cbir.1995.1106.
In standard culture conditions, three human hepatoma cell lines, Hep3B, PLC/PRF/5 and HepG2, were characterised by a predominant transcription of only two (H2 and L) among the four genes involved in the synthesis of inter-alpha-trypsin inhibitor (ITI)-related proteins. Pulse-chase experiments followed by immuno-precipitation with specific anti-L and anti-H ITI antisera showed that the proteins synthesised displayed a restricted L and/or H2 antigenic reactivity. Furthermore, while Hep3B and PLC/PRF/5 lines only synthesised ITI precursors (mainly the L-form), HepG2 cells were able to secrete an ITI-like protein. Immunocytochemical analyses substantiated these results with uneven distribution of heavy and light-chain polypeptide reactivity among the cells. The use of hepatoma cell models for the study of protein synthesis and assembly must therefore be considered cautiously.
在标准培养条件下，三种人肝癌细胞系，即Hep3B、PLC/PRF/5和HepG2，其特征在于，在参与α-胰蛋白酶抑制剂（ITI）相关蛋白合成的四个基因中，只有两个基因（H2和L）的转录占主导地位。脉冲追踪实验，随后用特异性抗L和抗H ITI抗血清进行免疫沉淀，结果表明，合成的蛋白质表现出有限的L和/或H2抗原反应性。此外，虽然Hep3B和PLC/PRF/5细胞系只合成ITI前体（主要是L型），但HepG2细胞能够分泌一种类似ITI的蛋白。免疫细胞化学分析证实了这些结果，细胞中重链和轻链多肽反应性分布不均。因此，在研究蛋白质合成和组装时，必须谨慎考虑使用肝癌细胞模型。
