从具有脂肪动和高海藻糖血症活性的周期蝉心体中分离出的一种新型神经肽的一级结构。

Primary structure of a novel neuropeptide isolated from the corpora cardiaca of periodical cicadas having adipokinetic and hypertrehalosemic activities.

作者信息

Raina A, Pannell L, Kochansky J, Jaffe H

机构信息

Insect Neurobiology and Hormone Laboratory, USDA, Beltsville, MD 20705, USA.

出版信息

Insect Biochem Mol Biol. 1995 Sep;25(8):929-32. doi: 10.1016/0965-1748(95)00032-q.

DOI:10.1016/0965-1748(95)00032-q

PMID:7550248

Abstract

A new neuropeptide hormone was isolated from the corpora cardiaca of the periodical cicadas, Magicicada species. Primary structure of the peptide as determined by a combination of automated Edman degradation after enzymatic deblocking with pyroglutamate aminopeptidase and mass spectrometry is: pGlu-Val-Asn-Phe-Ser-Pro-Ser-Trp-Gly-Asn-NH2. Synthetic peptide assayed in the green stink bug Nezara viridula caused a 112% increase in hemolymph lipids at a dose of 0.625 pmol, and a 67% increase in hemolymph carbohydrates at a dose of 2.5 pmol. Based on these results we designate this peptide, a first from order Homoptera, as Magicicada species-adipokinetic hormone (Mcsp-AKH).

摘要

一种新的神经肽激素是从周期蝉（魔蝉属物种）的心侧体中分离出来的。该肽的一级结构通过焦谷氨酸氨肽酶酶解去封闭后自动Edman降解和质谱联用确定为：焦谷氨酸-缬氨酸-天冬酰胺-苯丙氨酸-丝氨酸-脯氨酸-丝氨酸-色氨酸-甘氨酸-天冬酰胺-氨基。在绿蝽中检测合成肽，剂量为0.625皮摩尔时血淋巴脂质增加112%，剂量为2.5皮摩尔时血淋巴碳水化合物增加67%。基于这些结果，我们将这种肽（同翅目首个此类肽）命名为魔蝉属物种脂肪动激素（Mcsp-AKH）。