Shkap V, Pipano E, Zwernemann B
Department of Parasitology, Kimron Veterinary Institute, Bet Dagan, Israel.
Vet Res. 1995;26(4):328-34.
Besnoitia besnoiti multiplication in vitro was inhibited by a specific organelle complex-directed monoclonal antibody (MoAb) raised against the endozoite stage. Multiplication rates in antibody-treated cultures were lower at parasite/cell ratios of 1:1, 1:2 and 1:10, than in untreated cultures, after 4 or 8 d of incubation. At 1:100 ratio, there was no difference between test and control cultures irrespective of the incubation period. In in vivo experiments, the anti-B besnoiti MoAb had no neutralizing effect on the infectivity of endozoites. Inoculation of gerbils with antibody-preincubated endozoites, followed by treatment with these MoAb through 5 successive days ultimately failed to prevent death in any of them. In Western blots the specific anti-B besnoiti MoAb of the IgG1 subclass recognized a 70 kDa endozoite protein in the cytosolic and the insoluble membrane fraction of endozoites.
针对内子孢子阶段产生的一种特定细胞器复合物导向单克隆抗体（MoAb）可抑制贝氏贝诺孢子虫在体外的增殖。在1:1、1:2和1:10的寄生虫/细胞比例下，抗体处理培养物中的增殖率在孵育4天或8天后低于未处理的培养物。在1:100的比例下，无论孵育期如何，测试培养物和对照培养物之间均无差异。在体内实验中，抗贝氏贝诺孢子虫MoAb对内子孢子的感染性没有中和作用。用抗体预孵育的内子孢子接种沙鼠，随后连续5天用这些MoAb进行治疗，最终未能防止任何一只沙鼠死亡。在蛋白质免疫印迹中，IgG1亚类的特异性抗贝氏贝诺孢子虫MoAb在细胞质和内子孢子的不溶性膜部分中识别出一种70 kDa的内子孢子蛋白。
