Vijayalakshmi V, Gupta P D
Centre for Cellular and Molecular Biology, Hyderabad, India.
Epithelial Cell Biol. 1994;3(4):168-74.
We report a novel association of the calcium dependent cross-linking enzyme, transglutaminase (TGase) with the urea soluble reconstituted keratin filaments (RKF) isolated from the rat vaginal epithelial cells (VEC). This was ascertained by measuring the activity using 14C-spermidine incorporation and also by an increase in keratin filament aggregation by the addition of only TGase cofactor-calcium. These events were specifically inhibited by the treatment of calcium chelator, EDTA at a concentration > 2 mM as well as by pretreating the RKF with histamine, a TGase substrate inhibitor. The association was also exemplified by immunoblotting analysis where a specific and preferential polypeptide of molecular weight 58 kDa cross-reacted with TGase antibody amongst the other keratins. This phenomenon was not seen in the keratins isolated from skin, a non-targeting tissue for estradiol action.
我们报告了一种新的关联，即钙依赖性交联酶转谷氨酰胺酶（TGase）与从大鼠阴道上皮细胞（VEC）分离的尿素可溶性重组角蛋白丝（RKF）之间的关联。这是通过使用14C-亚精胺掺入来测量活性以及仅通过添加TGase辅因子钙来增加角蛋白丝聚集来确定的。这些事件在浓度> 2 mM的钙螯合剂EDTA处理以及用TGase底物抑制剂组胺预处理RKF时受到特异性抑制。这种关联也通过免疫印迹分析得到例证，其中分子量为58 kDa的特定且优先的多肽在其他角蛋白中与TGase抗体发生交叉反应。在从皮肤分离的角蛋白中未观察到这种现象，皮肤是雌二醇作用的非靶向组织。
