Baker P R, Gardner J J, Wilkinson D
Clinical Innovation Limited, Kenilworth, Warwickshire, UK.
J Chromatogr B Biomed Appl. 1995 Jun 9;668(1):59-65. doi: 10.1016/0378-4347(95)00049-o.
An automated HPLC method is described for the determination of nedocromil sodium in human urine. An HPLC autosampler is used to inject urine samples onto a short reversed-phase column. This column acts as a concentration column and performs a preliminary extraction. The concentration column is automatically back-flushed onto an ion-exchange column where final separation of nedocromil sodium from urine constituents occurs. Recovery, accuracy, precision, sensitivity and specificity were investigated. The method has been applied to urine samples from clinical studies, and the results were compared to those obtained using a radioimmunoassay developed previously.
描述了一种用于测定人尿中奈多罗米钠的自动高效液相色谱法。使用高效液相色谱自动进样器将尿液样品注入短反相柱。该柱作为浓缩柱，进行初步萃取。浓缩柱自动反冲到离子交换柱上，在此奈多罗米钠与尿液成分发生最终分离。研究了回收率、准确度、精密度、灵敏度和特异性。该方法已应用于临床研究的尿液样品，并将结果与先前开发的放射免疫分析法所得结果进行比较。
