人类（15P）异染色质染色体区域在核仁形成过程中的拓扑变化。

Topographic changes in a heterochromatic chromosome block in humans (15P) during formation of the nucleolus.

作者信息

Kaplan F S, O'Connor J P

机构信息

Department of Orthopaedics, Hospital of the University of Pennsylvania, Philadelphia 19104, USA.

出版信息

Chromosome Res. 1995 Aug;3(5):309-14. doi: 10.1007/BF00713069.

DOI:10.1007/BF00713069

PMID:7551545

Abstract

Fluorescence in situ hybridization and multispectral confocal laser scanning microscopy revealed a highly dynamic nucleolar-associated chromosome 15 satellite III heterochromatin cluster in humans. This nucleolar-associated DNA was highly decondensed at the metaphase plate compared with its topography at interphase and appeared to act as a centre for the post-mitotic reorganization of the nucleolus. Our data showed unexpected trans-mitotic changes in the topography of this nucleolar-associated satellite III DNA that suggest that this locus-specific heterochromatin superstructure may be involved in nucleolar organization.

摘要

荧光原位杂交和多光谱共聚焦激光扫描显微镜显示，人类中存在一个高度动态的与核仁相关的15号染色体卫星III异染色质簇。与间期时的拓扑结构相比，这种与核仁相关的DNA在中期板处高度解聚，并且似乎充当核仁有丝分裂后重新组织的中心。我们的数据显示了这种与核仁相关的卫星III DNA拓扑结构中意外的有丝分裂传递变化，这表明这种位点特异性异染色质超结构可能参与核仁组织。