免疫反应性雄激素受体水平降低并非孤立性尿道下裂的病因。
Decreased immunoreactive androgen receptor levels are not the cause of isolated hypospadias.
作者信息
Bentvelsen F M, Brinkmann A O, van der Linden J E, Schröder F H, Nijman J M
机构信息
Department of Pediatric Urology, Sophia Children's Hospital, Erasmus University of Rotterdam, The Netherlands.
出版信息
Br J Urol. 1995 Sep;76(3):384-8. doi: 10.1111/j.1464-410x.1995.tb07719.x.
OBJECTIVE
To elucidate whether diminished levels of androgen receptor (AR) are the underlying cause for the development of hypospadias by determining AR levels in the foreskins of boys with hypospadias.
PATIENTS AND METHODS
The content of AR was determined by a sensitive immunoblotting technique in extracts from preputial tissue obtained from 15 patients (mean age 2.5, SD +/- 1.5 years) with hypospadias and from seven controls (mean age 2.5, SD +/- 1.5 years). Immunoreactivity of the protein was measured by densitometry.
RESULTS
No significant difference in mean AR content was found between those boys with hypospadias (2.1 +/- 0.9 fmol/mg protein, +/- SD) and the age-matched control group (2.2 +/- 0.3 fmol/mg protein). Stratification by the severity of hypospadias (distal and proximal on the shaft, and penoscrotal hypospadias) showed that the severity was not related to tissue AR levels.
CONCLUSION
It is unlikely that hypospadias is caused by a decreased expression of AR.
目的
通过测定尿道下裂患儿包皮中雄激素受体（AR）水平，阐明AR水平降低是否为尿道下裂发生的潜在原因。
患者与方法
采用灵敏的免疫印迹技术，测定了15例尿道下裂患者（平均年龄2.5岁，标准差±1.5岁）和7例对照者（平均年龄2.5岁，标准差±1.5岁）包皮组织提取物中AR的含量。通过光密度测定法测量该蛋白的免疫反应性。
结果
尿道下裂患儿（2.1±0.9 fmol/mg蛋白，±标准差）与年龄匹配的对照组（2.2±0.3 fmol/mg蛋白）之间的平均AR含量无显著差异。按尿道下裂严重程度（阴茎体远端和近端以及阴茎阴囊型尿道下裂）分层显示，严重程度与组织AR水平无关。
结论
尿道下裂不太可能由AR表达降低引起。
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