胆碱能促分泌物质对食用蛙哈德氏腺形态的影响

Effect of cholinergic secretagogue substances on the morphology of the harderian gland in the frog, Rana esculenta.

作者信息

Di Matteo L, Baccari G C, Minucci S

机构信息

Dipartimento di Fisiologia Umana e Funzioni Biologiche Integrate F. Bottazzi, Italy.

出版信息

Comp Biochem Physiol A Physiol. 1995 Sep;112(1):29-34. doi: 10.1016/0300-9629(95)00095-o.

DOI:10.1016/0300-9629(95)00095-o

PMID:7553334

Abstract

The present study using secretagogue substances was undertaken to investigate Harderian gland secretion in the frog, Rana esculenta. Carbamylcholine chloride and bethanechol injections caused enhancement of the secretory activity and hyperemia, while nicotine did not. Morphological examinations showed reduced cellular height and dilated alveolar lumina, containing secretory granule discharge, nuclei and cytoplasmic fragments, indicating an apocrine and holocrine secretion type. The administration of atropine prevented the enhancement of the secretion. Our data suggest that cholinergic stimulation provokes enhancement of the secretory activity on the frog HG, and this mechanism appears to be mediated by the activation of the muscarinic receptors.

摘要

本研究使用促分泌物质来研究食用蛙（Rana esculenta）的哈德氏腺分泌情况。注射氯化氨甲酰胆碱和氨甲酰甲胆碱可导致分泌活性增强和充血，而尼古丁则不会。形态学检查显示细胞高度降低，肺泡腔扩张，含有分泌颗粒排出物、细胞核和细胞质碎片，表明为顶浆分泌和全浆分泌类型。阿托品的给药可阻止分泌增强。我们的数据表明，胆碱能刺激可激发食用蛙哈德氏腺分泌活性增强，且该机制似乎是由毒蕈碱受体的激活介导的。