De Sevilla L, McCune S A, White M E
Department of Animal Science, Ohio State University, Columbus 43210, USA.
Comp Biochem Physiol B Biochem Mol Biol. 1994 Oct-Nov;109(2-3):371-82. doi: 10.1016/0305-0491(94)90020-5.
No differences were detected in serum IGF-I levels between lean and obese male SHFF/Mcc-fa(cp) rats expressing non-insulin-dependent diabetes mellitus (NIDDM). In contrast, serum insulin levels, and blood glucose levels were significantly elevated in obese as compared to lean littermates (P < 0.05), indicating that the obese animals were diabetic. Northern blot analysis of total tissue RNA using labeled cDNAs for IGF-I and IGF-II revealed a decrease in liver and adipose IGF-I mRNA expression in the obese littermates while IGF-II mRNA expression was decreased only in adipose tissue of obese animals as compared to lean littermates.
在表现出非胰岛素依赖型糖尿病（NIDDM）的瘦型和肥胖型雄性SHFF/Mcc-fa(cp)大鼠之间，未检测到血清胰岛素样生长因子-I（IGF-I）水平存在差异。相比之下，与瘦型同窝仔鼠相比，肥胖型大鼠的血清胰岛素水平和血糖水平显著升高（P < 0.05），表明肥胖动物患有糖尿病。使用IGF-I和IGF-II的标记cDNA对总组织RNA进行Northern印迹分析显示，与瘦型同窝仔鼠相比，肥胖型同窝仔鼠的肝脏和脂肪组织中IGF-I mRNA表达降低，而IGF-II mRNA表达仅在肥胖动物的脂肪组织中降低。
