Ortona E, Margutti P, De Luca A, Tamburrini E, Visconti E, Siracusano A
Department of Immunology, Istituto Superiore di Sanità, Roma, Italy.
New Microbiol. 1995 Jul;18(3):335-40.
To gain further insight into the genetic variability between different isolates of Pneumocystis carinii of rat and human origin, we sequenced three DNA regions from the mitochondrial rRNA gene, the 5S rRNA gene and the dihydrofolate reductase gene (DHFR) of microorganisms isolated from three different immunosuppressed rat sources and from one HIV + patient with P. carinii pneumonia. Some point mutations and deletions were found among rat isolates in two regions (mitochondrial rRNA gene and 5S rRNA gene). Nucleotide sequence variations in these conserved regions support the hypothesis of the existence of several Pneumocystis strains infecting the same host species.
为了更深入了解源自大鼠和人类的不同卡氏肺孢子虫分离株之间的遗传变异性，我们对来自三种不同免疫抑制大鼠来源以及一名患有卡氏肺孢子虫肺炎的HIV阳性患者所分离出的微生物的线粒体rRNA基因、5S rRNA基因和二氢叶酸还原酶基因（DHFR）的三个DNA区域进行了测序。在大鼠分离株的两个区域（线粒体rRNA基因和5S rRNA基因）中发现了一些点突变和缺失。这些保守区域中的核苷酸序列变异支持了存在几种感染同一宿主物种的卡氏肺孢子虫菌株这一假说。
