Gaál K, Siklós J, Mózes T, Fejes Tóth G
Acta Physiol Acad Sci Hung. 1978;51(3):305-14.
The effect of papaverine on renal function and renin release was investigated in dogs in vivo and in vitro. Intrarenal arterial infusion of 0.1 mg/kg/min of papaverine for 10 minutes caused a significant rise in renal blood flow, a significant decrease in renal vascular resistance, clearance and extraction ratio of creatinine and PAH and in the amount of filtered sodium, without altering arterial blood pressure. There was a significant increase in sodium excretion and in the excreted percentage of filtered sodium (TRFNa). Renin activity (PRA) of arterial blood and renal venous blood, veno-arterious PRA-difference and renin secretion increased significantly after papaverine infusion. In order to eliminate the effect of hemodynamic changes on renin secretion, the effect of papaverine (10(-5), 10(-4)M) was investigated in vitro in surviving canine kidney cortex slices. Papaverine caused a significant increase in renin release and in tissue cAMP concentration. This supports the assumption that the increase in renin secretion might be due to a direct effect on the juxtaglomerular apparatus, by blocking phosphodiesterase activity and by increasing the renal cAMP level.
在犬体内和体外研究了罂粟碱对肾功能和肾素释放的影响。肾内动脉以0.1mg/kg/min的速度输注罂粟碱10分钟，可使肾血流量显著增加，肾血管阻力、肌酐和对氨基马尿酸的清除率及提取率以及滤过钠量显著降低，而动脉血压无改变。钠排泄量及滤过钠排泄百分比（TRFNa）显著增加。输注罂粟碱后，动脉血和肾静脉血的肾素活性（PRA）、肾静脉-动脉PRA差值及肾素分泌均显著增加。为消除血流动力学变化对肾素分泌的影响，在体外对存活的犬肾皮质切片研究了罂粟碱（10⁻⁵、10⁻⁴M）的作用。罂粟碱可使肾素释放及组织环磷酸腺苷（cAMP）浓度显著增加。这支持了肾素分泌增加可能是由于对肾小球旁器的直接作用，通过阻断磷酸二酯酶活性并增加肾cAMP水平的假设。
