Airoldi L, Magagnotti C, Chiappetta L, Bonfanti M, Pastorelli R, Fanelli R
Laboratory of Molecular Toxicology, Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
Carcinogenesis. 1995 Sep;16(9):2247-50. doi: 10.1093/carcin/16.9.2247.
A sensitive and specific method has been developed for the simultaneous analysis of different O6-alkylguanines. The cross-reactivity of two different antibodies raised against O6-methylguanosine and O6-butylguanosine for a series of O6-alkylguanines was exploited for the immunoaffinity purification of biological samples before quantitative analysis by gas chromatography/mass spectrometry. The method can be applied to the detection of O6-alkylguanines in DNA and appears to be useful for studying chemical carcinogen mechanisms in animals and possibly for the detection of human exposure to alkylating agents.
已开发出一种灵敏且特异的方法，用于同时分析不同的O6-烷基鸟嘌呤。利用针对O6-甲基鸟苷和O6-丁基鸟苷产生的两种不同抗体对一系列O6-烷基鸟嘌呤的交叉反应性，在通过气相色谱/质谱进行定量分析之前，对生物样品进行免疫亲和纯化。该方法可用于检测DNA中的O6-烷基鸟嘌呤，似乎对研究动物体内化学致癌物机制以及可能检测人类接触烷基化剂情况很有用。
