结肠上皮细胞系HT29克隆19A中细胞内pH的调节

Regulation of intracellular pH in the colonic epithelial cell line HT29 clone 19A.

作者信息

Busche R, Bartels J, von Engelhardt W

机构信息

Department of Physiology, School of Veterinary Medicine Hannover.

出版信息

Dtsch Tierarztl Wochenschr. 1995 Apr;102(4):161-2.

PMID:7555696

Abstract

Intracellular pH (pHi) of the colonic tumor cell line HT29 cl. 19A was studied by microspectrofluorometry using the pH-sensitive dye BCECF. Single cells within a confluent monolayer grown in a polarized manner on permeable supports were examined. An amiloride-sensitive Na(+)-H+ exchange and a stilbene-insensitive Cl(-)-HCO3- exchange mechanism have been identified in the basolateral membrane. This Na(+)-H+ exchange mechanism in the basolateral membrane is an acid extruder, whereas the Cl(-)-HCO3- exchanger is an acid loader. Both of these in the basolateral membrane located mechanisms are important for the maintenance of intracellular pH in HT29 cl. 19A cells.

摘要

使用pH敏感染料BCECF，通过显微分光荧光测定法研究了结肠肿瘤细胞系HT29 cl. 19A的细胞内pH（pHi）。对在可渗透支持物上以极化方式生长的汇合单层内的单细胞进行了检查。已在基底外侧膜中鉴定出一种amiloride敏感的Na(+)-H+交换和一种对芪不敏感的Cl(-)-HCO3-交换机制。基底外侧膜中的这种Na(+)-H+交换机制是一种酸排出器，而Cl(-)-HCO3-交换器是一种酸加载器。位于基底外侧膜中的这两种机制对于维持HT29 cl. 19A细胞的细胞内pH都很重要。